Difference between revisions of "Part:BBa K615000"
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<partinfo>BBa_K615000 short</partinfo>
 
<partinfo>BBa_K615000 short</partinfo>
  
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This strain is designed to test zinc finger binding using a one-hybrid metabolic selection system.  The endogenous genes HisB and PyrF have been knocked out and replaced with the yeast versions His3 and URA3 under the control of a Zif268 binding site.  The gene coding for the omega subunit of RNA polymerase (rpoZ) was knocked out by replacing it with a Zeocin cassette, and the strain is designed to be used in conjunction with BBa_K615001 (zinc finger-omega subunit expression construct).
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This strain is designed to test zinc finger binding using a one-hybrid metabolic selection system.  The endogenous genes HisB and PyrF have been knocked out and replaced with the yeast versions His3 and URA3 under the control of a Zif268 binding site.  The gene coding for the omega subunit of RNA polymerase (rpoZ) was knocked out by replacing it with a Zeocin cassette, and the strain is designed to be used in conjunction with a zinc finger-omega subunit expression construct.
  
 
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Revision as of 15:24, 25 September 2011

E. coli strain for His3-URA3 one-hybrid selection system

This strain is designed to test zinc finger binding using a one-hybrid metabolic selection system. The endogenous genes HisB and PyrF have been knocked out and replaced with the yeast versions His3 and URA3 under the control of a Zif268 binding site. The gene coding for the omega subunit of RNA polymerase (rpoZ) was knocked out by replacing it with a Zeocin cassette, and the strain is designed to be used in conjunction with a zinc finger-omega subunit expression construct.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
    Illegal PstI site found at 1762
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
    Illegal PstI site found at 1762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 845
    Illegal BglII site found at 965
    Illegal BglII site found at 1478
    Illegal BglII site found at 1538
    Illegal BamHI site found at 1768
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
    Illegal PstI site found at 1762
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
    Illegal PstI site found at 1762
    Illegal NgoMIV site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2458
    Illegal SapI site found at 121
    Illegal SapI site found at 331
    Illegal SapI.rc site found at 2305


Functional Parameters

HARVstrainsummary.png