Difference between revisions of "Part:BBa K560000"

Revision as of 13:37, 25 September 2011

Blue Light Sensor+PtrpL+mRFP1

LovTAP Photosensor is a chimeric protein. It is built by fusing LOV domain, which senses blue light, to the trpR-DNA binding domain of the transcription factor trp repressor. Under the presence of blue light, maximum at 450nm, the absorption of a photon leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 918
Illegal SpeI site found at 926
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 918
Illegal SpeI site found at 926
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 918
Illegal SpeI site found at 926
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 918
Illegal SpeI site found at 926
Illegal AgeI site found at 1525
Illegal AgeI site found at 1637
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 595

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K560000 short</partinfo>
-LovTAP Photosensor is a chimeric protein, which consists of LOV domain which senses blue light. It fused to the trpR-DNA binding domain of the transcription factor trp repressor.
+LovTAP Photosensor is a chimeric protein. It is built by fusing LOV domain, which senses blue light, to the trpR-DNA binding domain of the transcription factor trp repressor.
 Under the presence of blue light, maximum at 450nm, the absorption of a photon leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.