Difference between revisions of "Part:BBa K530029"
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| − | Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and TRP1 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. | + | Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and TRP1 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector. |
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Latest revision as of 01:42, 25 September 2011
pRSBB404 TRP1 Integrating Yeast Shuttle Vector
Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and TRP1 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1566
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3246
Illegal SapI site found at 2163
Plasmid Map
