Difference between revisions of "Part:BBa K530028"
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<partinfo>BBa_K530028 short</partinfo> | <partinfo>BBa_K530028 short</partinfo> | ||
| − | Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and HIS3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. | + | Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and HIS3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector. |
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Latest revision as of 01:41, 25 September 2011
pRSBB403 HIS3 Integrating Yeast Shuttle Vector
Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1008
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 899
Illegal BglII site found at 959 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1748
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3428
Illegal SapI site found at 2345
Plasmid Map
