Difference between revisions of "Part:BBa K530039"

Latest revision as of 01:19, 25 September 2011

pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 5186
Illegal PstI site found at 395
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 395
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 5186
Illegal PstI site found at 395
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 5186
Illegal PstI site found at 395
Illegal NgoMIV site found at 1676
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1085
Illegal BsaI.rc site found at 3356
Illegal SapI site found at 2273
Illegal SapI.rc site found at 932

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 __NOTOC__
 <partinfo>BBa_K530039 short</partinfo>
-URA3
+Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and URA3 selectable marker for ''S. cerevisiae''.  Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 16: @@
 <partinfo>BBa_K530039 parameters</partinfo>
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+===Plasmid Map===
+[[Image:pRSBB426.png]]