Difference between revisions of "Part:BBa K611009"

 
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<partinfo>BBa_K611009 short</partinfo>
 
<partinfo>BBa_K611009 short</partinfo>
  
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This part is used to characterize mutants of BBa_C0040 (TetR). By adding a mutant of BBa_C0040 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.
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This part is used to characterize mutants of BBa_C0040 (TetR). A mutant of BBa_C0040 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI PstI. One can then measure several of the mutant's properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 01:15, 25 September 2011

TetR Characterization Construct

This part is used to characterize mutants of BBa_C0040 (TetR). A mutant of BBa_C0040 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI PstI. One can then measure several of the mutant's properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1070
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724