Difference between revisions of "Part:BBa K530034"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K530034 short</partinfo>
 
<partinfo>BBa_K530034 short</partinfo>
−
 
+
Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and LEU2 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
−
LEU2
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 17: Line 15:
 
<partinfo>BBa_K530034 parameters</partinfo>
 
<partinfo>BBa_K530034 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===Plasmid Map===
 +
[[Image:pRS415.png]]

Revision as of 00:51, 25 September 2011

pRSBB415 LEU2 CEN/ARS Yeast Shuttle Vector Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and LEU2 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4479
    Illegal SapI site found at 3396
    Illegal SapI site found at 5500


Plasmid Map

PRS415.png