Difference between revisions of "Part:BBa K530033"

 
 
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TRP1
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Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and TRP1 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
  
 
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===Plasmid Map===
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[[Image:pRS414.png]]

Latest revision as of 00:45, 25 September 2011

pRSBB414 TRP1 CEN/ARS Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and TRP1 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3246
    Illegal SapI site found at 2163
    Illegal SapI site found at 4267


Plasmid Map

PRS414.png