Difference between revisions of "Part:BBa K590022:Design"
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===Design Notes=== | ===Design Notes=== | ||
Point mutation at residues 319, 358, and 368 from Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively. | Point mutation at residues 319, 358, and 368 from Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 23:26, 24 September 2011
Kumamolisin-As_G319S, D358G, D368H
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1696
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 447
Illegal NgoMIV site found at 720
Illegal NgoMIV site found at 1248
Illegal NgoMIV site found at 1494
Illegal AgeI site found at 772
Illegal AgeI site found at 1348
Illegal AgeI site found at 1588 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Point mutation at residues 319, 358, and 368 from Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.
Source
In-House Mutant