Difference between revisions of "Part:BBa K590024:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Point | + | Point mutations at residues 354, 358, and 368 from Serine to Asparagine, Aspartate to Glycine, and Aspartate to Histidine, respectively. |
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===Source=== | ===Source=== | ||
Revision as of 23:26, 24 September 2011
Kumamolisin-As_S354N, D358G, D368H
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1696
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 447
Illegal NgoMIV site found at 720
Illegal NgoMIV site found at 1248
Illegal NgoMIV site found at 1494
Illegal AgeI site found at 772
Illegal AgeI site found at 1348
Illegal AgeI site found at 1588 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Point mutations at residues 354, 358, and 368 from Serine to Asparagine, Aspartate to Glycine, and Aspartate to Histidine, respectively.
Source
In-House Mutant