Difference between revisions of "Part:BBa K590034"
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<partinfo>BBa_K590034 short</partinfo>
 
<partinfo>BBa_K590034 short</partinfo>
  
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This part encodes FabH2. [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] has attempted to produce branched alkanes using this part and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the Petrobrick].  
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This part encodes FabH2. [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] has attempted to produce branched alkanes using this part and the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 Petrobrick].  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 23:19, 24 September 2011

FabH2

This part encodes FabH2. [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] has attempted to produce branched alkanes using this part and the Petrobrick.

Usage and Biology

FabH2 is from Bacillus subtilis. The FabH family of proteins initiates fatty acid elongation by converting an Acyl-CoA into an Acyl-ACP, with is extended by 2 carbon units to form longer chain length fatty acids. Normally, FabH proteins use a simple 2-carbon acetyl-CoA to start fatty acid biosynthesis, resulting in linear fatty acids. However, FabH2 can also use Isobutyryl-CoA, Isovaleryl-CoA, and 2-Methylbutyryl-CoA (products from Valine, Leucine, and Isoleucine degredation, resulting in 2-methyl branched fatty acid production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 310
    Illegal AgeI site found at 949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355