Difference between revisions of "Part:BBa K592100:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K592100=== | ===Applications of BBa_K592100=== | ||
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| + | [[Image:Promoter characterization.png]] | ||
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| + | '''iGEM11_Uppsala-Sweden'''. BFP used as reporter. This part has been successfully used as a reporter gene for promoter characterization using flow cytometry. The excitation peak at 399 nm makes it well suited for use in flow cytometers using a 407 nm violet laser such as the FACSAria II, and the emission peak at 456 works well with a DAPI filter (band pass 450/40). The Y axis measures the promoter activity in RPU, relative promoter units, where the activity of the J23101 promoter is defined as 1 RPU. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 22:53, 24 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K592100
iGEM11_Uppsala-Sweden. BFP used as reporter. This part has been successfully used as a reporter gene for promoter characterization using flow cytometry. The excitation peak at 399 nm makes it well suited for use in flow cytometers using a 407 nm violet laser such as the FACSAria II, and the emission peak at 456 works well with a DAPI filter (band pass 450/40). The Y axis measures the promoter activity in RPU, relative promoter units, where the activity of the J23101 promoter is defined as 1 RPU.
User Reviews
UNIQ5d59c8eb1d5da123-partinfo-00000000-QINU UNIQ5d59c8eb1d5da123-partinfo-00000001-QINU