Difference between revisions of "Part:BBa K590052"

Revision as of 21:37, 24 September 2011

Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) Fusion

This part encodes a direct gene fusion product of the two enzymes, Aldehyde Decarbonylase (ADC) and Acyl-ACP Reductase(AAR), in a linear configuration with a glycine-serine linker for co-localization.

Usage and Biology

We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production. For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].

Washington2011 Alkane Production Localization Graph1.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590052 short</partinfo>
-This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase(AAR)], in a linear configuration with a glycine-serine linker for co-localization. For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].
+This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase(AAR)], in a linear configuration with a glycine-serine linker for co-localization.
 ===Usage and Biology===
-We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production.
+We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production. For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].
 [[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |center]]