Difference between revisions of "Part:BBa K575024"

Revision as of 19:51, 24 September 2011

When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.

LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0030), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 58
Illegal NheI site found at 921
Illegal NheI site found at 944
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1300
Illegal AgeI site found at 1497
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 830

Revision as of 19:50, 24 September 2011 (view source) Nirmitd7 (Talk \| contribs) ← Older edit		Revision as of 19:51, 24 September 2011 (view source) Nirmitd7 (Talk \| contribs) Newer edit →
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	[[Image:Standardized Fluorescence for S1.jpg]]__NOTOC__		[[Image:Standardized Fluorescence for S1.jpg]]__NOTOC__

−	When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control.	+	When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.