Difference between revisions of "Part:BBa K575024"
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[[Image:Standardized Fluorescence for S1.jpg]]__NOTOC__
 
[[Image:Standardized Fluorescence for S1.jpg]]__NOTOC__
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When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control.
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<partinfo>BBa_K575024 short</partinfo>
 
<partinfo>BBa_K575024 short</partinfo>
  

Revision as of 19:50, 24 September 2011

Standardized Fluorescence for S1.jpg

When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control.


LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0030), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 921
    Illegal NheI site found at 944
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1300
    Illegal AgeI site found at 1497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830