Difference between revisions of "Part:BBa K516212:Design"
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<li>The Biobrick Part was designed without a terminator element downstream the luxI coding sequence. <br> | <li>The Biobrick Part was designed without a terminator element downstream the luxI coding sequence. <br> | ||
Nevertheless, it is possible use this part into plasmid vectors (such as pSB4C5) containing a Terminator in their backbones. </li> | Nevertheless, it is possible use this part into plasmid vectors (such as pSB4C5) containing a Terminator in their backbones. </li> | ||
| − | <li><i>luxI</i> coding sequence is LVA tagged to decrease the protein half-life. (Andersen JB et al. 1998)</li> | + | <li><i>luxI</i> coding sequence is LVA tagged to decrease the protein half-life. (<i><b>Andersen JB</b> et al. 1998</i>)</li> |
</ul> | </ul> | ||
Latest revision as of 16:05, 24 September 2011
LuxI protein generator with Ptet and RBS B0032 (TERM-)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- The Biobrick Part was designed without a terminator element downstream the luxI coding sequence.
Nevertheless, it is possible use this part into plasmid vectors (such as pSB4C5) containing a Terminator in their backbones. - luxI coding sequence is LVA tagged to decrease the protein half-life. (Andersen JB et al. 1998)
Source
Registry