Difference between revisions of "Part:BBa K542007"
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The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression. | The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression. | ||
| − | Intermediate part to assemble the "Co-localization Construct". | + | Intermediate part to assemble the "Co-localization Testing Construct". |
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Revision as of 15:56, 24 September 2011
Unregulated TetR with pTet Regulated Arg-tagged ECFP
Made by assembling BBa_P0440 with BBa_K331033.
The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter (BBa_R0010) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.
Intermediate part to assemble the "Co-localization Testing Construct".
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]