Difference between revisions of "Part:BBa K516330:Design"
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The BioBrick parts were selected to reduce the internal homology, that could be cause of recombination or mutation events (Sleight SC et al. 2010). <br> | The BioBrick parts were selected to reduce the internal homology, that could be cause of recombination or mutation events (Sleight SC et al. 2010). <br> | ||
| − | The choise of Transcriptional terminator was done purposely: in fact the alligniment of the single terminator BBa_B1006 and the double terminator | + | The choise of Transcriptional terminator was done purposely: in fact the alligniment of the single terminator <partinfo>BBa_B1006</partinfo> and the double terminator <partinfo>BBa_B0015</partinfo> does not show a significant sequence homology. |
===Source=== | ===Source=== | ||
Revision as of 15:41, 24 September 2011
mRFP producer controlled by 3OC6-HSL with RBS B0030
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1553
Illegal AgeI site found at 1665 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 912
Design Notes
--
The BioBrick parts were selected to reduce the internal homology, that could be cause of recombination or mutation events (Sleight SC et al. 2010).
The choise of Transcriptional terminator was done purposely: in fact the alligniment of the single terminator BBa_B1006 and the double terminator BBa_B0015 does not show a significant sequence homology.
Source
Registry
References
Pasotti L, Quattrocelli M, Galli D et al. (2011) Multiplexing and demultiplexing logic functions for computing signal processing tasks in synthetic biology. Biotechnol. J. 6(7):784-95.