Difference between revisions of "Part:BBa K606035"

Latest revision as of 18:58, 23 September 2011

pT7 SpoVG GFP T7terminator.

T7 RNA polymease controlled GFP expression system for B. subtilis and E. coli

Usage and Biology

This part is the concatenation of the promoter pT7 (I719004) with the RBS SpoVG (K143021), that design is for B. subtilis also works in E. coli. This part works very efficiently and have been caracterized in E. coli BL21 cells. The part is in pSB1C3.

See the experiment page of this part or our wiki page[http://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion] for additional information

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 917
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 693

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-This part is the concatenation of the promoter pT7 (I719004) with the RBS SpoVG (K143021), that is design for B. subtilis but that also works in E. coli. This part works very efficiently and have been caracterized in E. coli BL21 cells. The part is in pSB1C3.
+This part is the concatenation of the promoter pT7 (I719004) with the RBS SpoVG (K143021), that design is for B. subtilis also works in E. coli. This part works very efficiently and have been caracterized in E. coli BL21 cells. The part is in pSB1C3.
 See the experiment page of this part or our wiki page[http://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion] for additional information