Difference between revisions of "Part:BBa K606025"
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<partinfo>BBa_K606025 short</partinfo> | <partinfo>BBa_K606025 short</partinfo> | ||
| − | YFP:tetR is a recombinant fusion protein. It is composed | + | YFP:tetR is a recombinant fusion protein. It is composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array (<partinfo>BBa_K606026</partinfo>) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.<br> <br>Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004) |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 17:59, 23 September 2011
YFP:tetR recombinant fusion protein
YFP:tetR is a recombinant fusion protein. It is composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array (BBa_K606026) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.
Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)
Usage and Biology
The combination of both parts K606025 and K606026 can be use to concentrate a fluorescence signal and make a smaller nmber of fluorescent proteins visible under the microscope. See the experiments page for characterization and picture of what can be seen under the microscope.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 631
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]