Difference between revisions of "Part:BBa K542002"
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Made by assembling [https://parts.igem.org/Part:BBa_K542000 BBa_K542000] with [https://parts.igem.org/Part:BBa_I13453 BBa_I13453]. | Made by assembling [https://parts.igem.org/Part:BBa_K542000 BBa_K542000] with [https://parts.igem.org/Part:BBa_I13453 BBa_I13453]. | ||
| − | Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. not arabinose inducible). <br> | + | Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible). <br> |
Intermediate part to assemble the "Co-localization Construct". | Intermediate part to assemble the "Co-localization Construct". | ||
Revision as of 17:31, 23 September 2011
Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)
Made by assembling BBa_K542000 with BBa_I13453.
Note that the pBAD promoter (BBa_I13453) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible).
Intermediate part to assemble the "Co-localization Construct".
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 735
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 675
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]