Difference between revisions of "Part:BBa K542002"

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Made by assembling [https://parts.igem.org/Part:BBa_K542000 BBa_K542000] with [https://parts.igem.org/Part:BBa_I13453 BBa_I13453].
 
Made by assembling [https://parts.igem.org/Part:BBa_K542000 BBa_K542000] with [https://parts.igem.org/Part:BBa_I13453 BBa_I13453].
  
Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. not arabinose inducible). <br>
+
Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible). <br>
 
Intermediate part to assemble the "Co-localization Construct".  
 
Intermediate part to assemble the "Co-localization Construct".  
  

Revision as of 17:31, 23 September 2011

Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)

Made by assembling BBa_K542000 with BBa_I13453.

Note that the pBAD promoter (BBa_I13453) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible).
Intermediate part to assemble the "Co-localization Construct".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]