Difference between revisions of "Part:BBa B0031:Experience"

Revision as of 16:13, 23 September 2011

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Please enter how you used this part and how it worked out.

Applications of BBa_B0031

User Reviews

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.

See Experience page of BBa_B0034 for more details.

Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter:

RBS	eff_{p_Lux}	eff_{p_Tet}	eff_J23101	Declared efficiency
B0030	0.40	1.6814	2.45	0,6
B0031	0.01	ND	0.04	0,07
B0032	0.19	0.4193	0.40	0,3
B0034	1	1	1	1

Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI:

RBS	eff_mRFP	eff_AiiA	eff_LuxI	Declared efficiency
B0030	1.72	0.96	0.45	0,6
B0031	0.03	0.78	0.028	0,07
B0032	0.37	0.51	N.D.	0.3
B0034	1	1	1	1

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@@ Line 52: / Line 52: @@
 <br><br>
-See <a href="https://parts.igem.org/Part:BBa_B0034:Experience">Experience page of BBa_B0034</a>for more details.
+See <a href="https://parts.igem.org/Part:BBa_B0034:Experience">Experience page of BBa_B0034</a> for more details.
 <br><br>
@@ Line 66: / Line 66: @@
 <td class='row'><b>Declared efficiency</b></td>
 </tr><tr>
-<td class='row'>B0031</td><td class='row'>0.40</td><td class='row'>1.6814</td><td class='row'>2.45</td><td class='row'>0,6</td>
+<td class='row'>B0030</td><td class='row'>0.40</td><td class='row'>1.6814</td><td class='row'>2.45</td><td class='row'>0,6</td>
 </tr><tr>
 <td class='row'>B0031</td><td class='row'>0.01</td><td class='row'>ND</td><td class='row'>0.04</td><td class='row'>0,07</td>
@@ Line 85: / Line 85: @@
 <td class='row'><b>Declared efficiency</b></td>
 </tr><tr>
-<td class='row'>B0031</td><td class='row'>1.72</td><td class='row'>0.96</td><td class='row'>0.45</td><td class='row'>0,6</td>
+<td class='row'>B0030</td><td class='row'>1.72</td><td class='row'>0.96</td><td class='row'>0.45</td><td class='row'>0,6</td>
 </tr><tr>
 <td class='row'>B0031</td><td class='row'>0.03</td><td class='row'>0.78</td><td class='row'>0.028</td><td class='row'>0,07</td>