Difference between revisions of "Part:BBa B0030:Experience"
| Line 33: | Line 33: | ||
The miniprep, single and double digests all worked. However we did not use this part for further cloning. | The miniprep, single and double digests all worked. However we did not use this part for further cloning. | ||
|} | |} | ||
| + | <!-- DON'T DELETE --><partinfo>BBa_B0030 EndReviews</partinfo> | ||
| + | |||
| + | |||
| + | <!-- DON'T DELETE --><partinfo>BBa_B0030 StartReviews</partinfo> | ||
| + | <!-- Template for a user review | ||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
| + | |width='10%'| | ||
| + | <partinfo>BBa_B0030 AddReview 4</partinfo> | ||
| + | <I>Username</I> | ||
| + | |width='60%' valign='top'| | ||
| + | Enter the review inofrmation here. | ||
| + | |}; | ||
| + | <!-- End of the user review template --> | ||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
| + | |width='10%'| | ||
| + | <partinfo>BBa_B0030 AddReview 5</partinfo> | ||
| + | <I>UNIPV-Pavia iGEM 2011</I> | ||
| + | |width='60%' valign='top'| | ||
| + | <html> | ||
| + | |||
| + | <em> | ||
| + | NB: unless differently specified, all tests were performed in <a href='http://2011.igem.org/Team:UNIPV-Pavia/Protocols#MG1655Z1'><em>E. coli</em> MGZ1</a> in M9 supplemented medium at 37°C in low copy plasmid <A HREF="https://parts.igem.org/wiki/index.php/Part:pSB4C5">pSB4C5</a>. | ||
| + | </em> | ||
| + | <br><br> | ||
| + | |||
| + | See <a href="https://parts.igem.org/Part:BBa_B0034:Experience">Experience page of BBa_B0034</a>for more details. | ||
| + | |||
| + | <br><br> | ||
| + | |||
| + | Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter: | ||
| + | <br><br> | ||
| + | |||
| + | <div align="center"><table class='data' width='70%'><tr> | ||
| + | <td class='row'><b>RBS</b></td> | ||
| + | <td class='row'><b>eff<sub>p<sub>Lux</sub></sub></b></td> | ||
| + | <td class='row'><b>eff<sub>p<sub>Tet</sub></sub></b></td> | ||
| + | <td class='row'><b>eff<sub>J23101</sub></b></td> | ||
| + | <td class='row'><b>Declared efficiency</b></td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0030</td><td class='row'>0.40</td><td class='row'>1.6814</td><td class='row'>2.45</td><td class='row'>0,6</td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0031</td><td class='row'>0.01</td><td class='row'>ND</td><td class='row'>0.04</td><td class='row'>0,07</td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0032</td><td class='row'>0.19</td><td class='row'>0.4193</td><td class='row'>0.40</td><td class='row'>0,3</td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0034</td><td class='row'>1</td><td class='row'>1</td><td class='row'>1</td><td class='row'>1</td> | ||
| + | </tr></table></div> | ||
| + | <br><br> | ||
| + | Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA (<em> E. coli</em> TOP10, high copy number plasmid) or LuxI: | ||
| + | <br><br> | ||
| + | |||
| + | <div align="center"><table class='data' width='70%'><tr> | ||
| + | <td class='row'><b>RBS</b></td> | ||
| + | <td class='row'><b>eff<sub>mRFP</sub></b></td> | ||
| + | <td class='row'><b>eff<sub>AiiA</sub></b></td> | ||
| + | <td class='row'><b>eff<sub>LuxI</sub></b></td> | ||
| + | <td class='row'><b>Declared efficiency</b></td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0030</td><td class='row'>1.72</td><td class='row'>0.96</td><td class='row'>0.45</td><td class='row'>0,6</td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0031</td><td class='row'>0.03</td><td class='row'>0.78</td><td class='row'>0.028</td><td class='row'>0,07</td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0032</td><td class='row'>0.37</td><td class='row'>0.51</td><td class='row'>N.D.</td><td class='row'>0.3</td> | ||
| + | </tr><tr> | ||
| + | <td class='row'>B0034</td><td class='row'>1</td><td class='row'>1</td><td class='row'>1</td><td class='row'>1</td> | ||
| + | </tr></table></div> | ||
| + | |||
| + | |||
| + | |||
| + | </html> | ||
| + | |} | ||
| + | |||
<!-- DON'T DELETE --><partinfo>BBa_B0030 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_B0030 EndReviews</partinfo> | ||
Revision as of 16:09, 23 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_B0030
User Reviews
UNIQba0f9d372129ef2d-partinfo-00000000-QINU
|
•••••
Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
|
•••
Aberdeen_Scotland 2009 |
The miniprep, single and double digests all worked. However we did not use this part for further cloning. |
UNIQba0f9d372129ef2d-partinfo-00000003-QINU
UNIQba0f9d372129ef2d-partinfo-00000004-QINU
|
•••••
UNIPV-Pavia iGEM 2011 |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.
Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI:
|
UNIQba0f9d372129ef2d-partinfo-00000007-QINU