Difference between revisions of "Part:BBa K516999"

 
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<partinfo>BBa_K516999 short</partinfo>
 
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When the SpeI-PstI DNA fragment from <partinfo>BBa_J61002</partinfo>, containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into <partinfo>pSB1C3</partinfo> (digested SpeI-PstI) the resulting plasmid is BBa_K516999. See <partinfo>pSB1C3</partinfo> for detailed feature information.
 
When the SpeI-PstI DNA fragment from <partinfo>BBa_J61002</partinfo>, containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into <partinfo>pSB1C3</partinfo> (digested SpeI-PstI) the resulting plasmid is BBa_K516999. See <partinfo>pSB1C3</partinfo> for detailed feature information.
  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:31, 23 September 2011

pSB1C3 with RBS-RFP-TT between SpeI-PstI

When the SpeI-PstI DNA fragment from BBa_J61002, containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into pSB1C3 (digested SpeI-PstI) the resulting plasmid is BBa_K516999. See pSB1C3 for detailed feature information.

<img alt="" src="BBa_I13507.jpg" class="thumbimage" width="50%"></a>

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2918
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 2924
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2918
    Illegal XhoI site found at 1902
    Illegal XhoI site found at 2794
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2918
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2918
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2933
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.