Difference between revisions of "Part:BBa C0061:Experience"
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| − | The four measurment parts <a href="http://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized#pTetLuxI">p<sub>tet</sub>-RBSx-LuxI</a> were quantitatively characetrized using the BBa_T9002 biosensor | + | The four measurment parts <a href="http://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized#pTetLuxI">p<sub>tet</sub>-RBSx-LuxI</a> were quantitatively characetrized using the BBa_T9002 biosensor.<br> |
| − | The HSL synthesis rate has been evaluated | + | The HSL synthesis rate has been evaluated, estimating V<sub>max</sub>, k<sub>M,LuxI</sub> and α<sub>RBSx</sub> parameters of the equations below:<br> |
| − | A significative HSL production was observed for <A HREF="https://parts.igem.org/wiki/index.php/Part:BBa_K516210">BBa_K516210</a>, <A HREF="https://parts.igem.org/wiki/index.php/Part:BBa_K516211">BBa_K516211</a> and <A HREF="https://parts.igem.org/wiki/index.php/Part:BBa_K516214">BBa_K516214</a>, while no HSL production was observed for <A HREF="https://parts.igem.orgwiki/index.php/Part:BBa_K516212">BBa_K516212</a>. The parameters V<sub>max</sub>, k<sub>M,LuxI</sub> and α<sub>RBSx</sub> were estimated with a simultaneous fitting of the data collected | + | <div style='text-align:justify'><div class="thumbinner" style="width: 850px;"> |
| + | <a href="https://static.igem.org/mediawiki/2011/a/a6/Pc_luxi.jpg"> | ||
| + | <img alt="" src="https://static.igem.org/mediawiki/2011/a/a6/Pc_luxi.jpg" class="thumbimage" height="70%" width="68%"></a></div></div> | ||
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| + | A significative HSL production was observed for <A HREF="https://parts.igem.org/wiki/index.php/Part:BBa_K516210">BBa_K516210</a>, <A HREF="https://parts.igem.org/wiki/index.php/Part:BBa_K516211">BBa_K516211</a> and <A HREF="https://parts.igem.org/wiki/index.php/Part:BBa_K516214">BBa_K516214</a>, while no HSL production was observed for <A HREF="https://parts.igem.orgwiki/index.php/Part:BBa_K516212">BBa_K516212</a>. The parameters V<sub>max</sub>, k<sub>M,LuxI</sub> and α<sub>RBSx</sub> were estimated with a simultaneous fitting of the data collected (HSL concentration measured in the supernatants of cultures, see <a href="https://parts.igem.org/Part:BBa_T9002:Experience#t9002">BBa_T9002 Experience page</a>): | ||
<div align="justify"><div class="thumbinner" style="width: 850px;"> | <div align="justify"><div class="thumbinner" style="width: 850px;"> | ||
Revision as of 14:26, 23 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_C0061
User Reviews
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Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
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wmholtz |
Using this part, I have successfully constructed and tested a quorum sensing circuit in E. coli. |
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Youri |
This part was used as a subpart in K546005, K546546 and found to be functional. |
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UNIQ4348b022ea103bdc-partinfo-00000005-QINU
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UNIPV-Pavia iGEM 2011 |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.
Though these parts don't have a transcriptional terminator, they have been characterized in low copy plasmid pSB4C5, that contains the BBa_B0054 terminator. The four measurment parts ptet-RBSx-LuxI were quantitatively characetrized using the BBa_T9002 biosensor. The HSL synthesis rate has been evaluated, estimating Vmax, kM,LuxI and αRBSx parameters of the equations below:  A significative HSL production was observed for BBa_K516210, BBa_K516211 and BBa_K516214, while no HSL production was observed for BBa_K516212. The parameters Vmax, kM,LuxI and αRBSx were estimated with a simultaneous fitting of the data collected (HSL concentration measured in the supernatants of cultures, see BBa_T9002 Experience page):  Parameters' values are summarized in the table below:
The provided parameters kM and Vmax represent the enzymatic activity of LuxI, described by our model. They must not be confused with the operative parameters of the Michaelis-Menten relation. |
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