Difference between revisions of "Part:BBa K516222:Experience"
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The AiiA enzyme activity has been characterized under the regulation of p<sub>tet</sub> promoter, assaying its enzymatic activity.<br> | The AiiA enzyme activity has been characterized under the regulation of p<sub>tet</sub> promoter, assaying its enzymatic activity.<br> | ||
| − | The four measurement parts <a href='http://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized#pTetAiiA'>p<sub>Tet</sub>-RBSx-AiiA-TT</a> were assayed by <a href=' | + | The four measurement parts <a href='http://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized#pTetAiiA'>p<sub>Tet</sub>-RBSx-AiiA-TT</a> were assayed by BBa_T9002 biosensor - see <a href='https://parts.igem.org/Part:BBa_T9002:Experience#t9002PV11'>experience page</a>. |
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No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. <br> | No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. <br> | ||
Latest revision as of 14:18, 23 September 2011
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Applications of BBa_K516222
User Reviews
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UNIPV-Pavia iGEM 2011 |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.
Characterized with:
The four measurement parts pTet-RBSx-AiiA-TT were assayed by BBa_T9002 biosensor - see experience page. No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. A significant HSL degradation was observed in E. coli TOP10 in high copy number plasmid pSB1A2. In this case, a significant difference in degradation between pTet-RBSx-AiiA-TT and the negative control was observed, also just after 7 hours. That was the proof of the good functioning (in HIGH COPY) of the enzyme.
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