Difference between revisions of "Part:BBa K516220:Experience"
(→User Reviews) |
(→User Reviews) |
||
| Line 52: | Line 52: | ||
The AiiA enzyme activity has been characterized under the regulation of p<sub>tet</sub> promoter, assaying its enzymatic activity.<br> | The AiiA enzyme activity has been characterized under the regulation of p<sub>tet</sub> promoter, assaying its enzymatic activity.<br> | ||
| − | The four measurement parts <a href='http://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized#pTetAiiA'>p<sub>Tet</sub>-RBSx-AiiA-TT</a> were assayed by <a href=' | + | The four measurement parts <a href='http://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized#pTetAiiA'>p<sub>Tet</sub>-RBSx-AiiA-TT</a> were assayed by <a href='https://parts.igem.org/Part:BBa_T9002:Experience#t9002PV11'>BBa_T9002 biosensor - experience page</a>. . |
<br> | <br> | ||
No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. <br> | No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. <br> | ||
Revision as of 14:14, 23 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K516220
User Reviews
UNIQc5bac99fbd6003f5-partinfo-00000000-QINU UNIQc5bac99fbd6003f5-partinfo-00000001-QINU
UNIQc5bac99fbd6003f5-partinfo-00000002-QINU
|
•••••
UNIPV-Pavia iGEM 2011 |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.
Characterized with:
The four measurement parts pTet-RBSx-AiiA-TT were assayed by BBa_T9002 biosensor - experience page. . No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. A significant HSL degradation was observed in E. coli TOP10 in high copy number plasmid pSB1A2. In this case, a significant difference in degradation between pTet-RBSx-AiiA-TT and the negative control was observed, also just after 7 hours. That was the proof of the good functioning (in HIGH COPY) of the enzyme.
|
UNIQc5bac99fbd6003f5-partinfo-00000005-QINU