Difference between revisions of "Part:BBa K516222:Experience"

 
(→‎User Reviews)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
Line 18: Line 17:
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 +
<!-- DON'T DELETE --><partinfo>BBa_K516222 EndReviews</partinfo>
 +
 +
 +
<!-- DON'T DELETE --><partinfo>BBa_K516222 StartReviews</partinfo>
 +
<!-- Template for a user review
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_K516222 AddReview 1</partinfo>
 +
<I>Username</I>
 +
|width='60%' valign='top'|
 +
Enter the review inofrmation here.
 +
|};
 +
<!-- End of the user review template -->
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_K516222 AddReview 5</partinfo>
 +
<I>UNIPV-Pavia iGEM 2011</I>
 +
|width='60%' valign='top'|
 +
<html>
 +
<em>
 +
NB: unless differently specified, all tests were performed in <a href='http://2011.igem.org/Team:UNIPV-Pavia/Protocols#MG1655Z1'><em>E. coli</em> MGZ1</a> in M9 supplemented medium at 37°C in low copy plasmid <A HREF="https://parts.igem.org/wiki/index.php/Part: pSB4C5">pSB4C5</a>.
 +
</em>
 +
<br>
 +
<br>
 +
<p align='justify'>
 +
Characterized with:
 +
<ol>
 +
<ul>
 +
<li> <A HREF="https://parts.igem.org/wiki/index.php/Part: BBa_K516220 "> BBa_K516220 </a> p<sub>Tet</sub>-RBS30-AiiA-TT </li>
 +
<li> <A HREF="https://parts.igem.org/wiki/index.php/Part: BBa_K516221 "> BBa_K516221 </a> p<sub>Tet</sub>-RBS31-AiiA-TT </li>
 +
<li> <A HREF="https://parts.igem.org/wiki/index.php/Part: BBa_K516224 "> BBa_K516224 </a> p<sub>Tet</sub>-RBS34-AiiA-TT </li></ul></ol>
 +
 +
The AiiA enzyme activity has been characterized under the regulation of p<sub>tet</sub> promoter, assaying its enzymatic activity.<br>
 +
The four measurement parts <a href='http://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized#pTetAiiA'>p<sub>Tet</sub>-RBSx-AiiA-TT</a> were assayed by <a href='http://2011.igem.org/Team:UNIPV-Pavia/Measurements#T9002'>BBa_T9002 biosensor</a>.
 +
<br>
 +
No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. <br>
 +
A significant HSL degradation was observed in <em>E. coli</em> TOP10 in high copy number plasmid pSB1A2. <br>
 +
In this case, a significant difference in degradation between p<sub>Tet</sub>-RBSx-AiiA-TT and the negative control was observed, also just after 7 hours. That was the proof of the good functioning (in HIGH COPY) of the enzyme.
 +
 +
 +
<table width='100%'><tr><td width='50%'>
 +
<div style="text-align:justify"><div class="thumbinner" width='100%'>
 +
<a href="https://static.igem.org/mediawiki/2011/6/6c/Aiia_HC_B0030.jpg">
 +
<img alt="" src="https://static.igem.org/mediawiki/2011/6/6c/Aiia_HC_B0030.jpg" class="thumbimage" width="100%"></a></div></div>
 +
</td>
 +
<td width='50%'>
 +
<div style="text-align:justify"><div class="thumbinner" width='100%'>
 +
<a href="https://static.igem.org/mediawiki/2011/e/ec/Aiia_HC_B0031.jpg">
 +
<img alt="" src="https://static.igem.org/mediawiki/2011/e/ec/Aiia_HC_B0031.jpg" class="thumbimage" width="100%"></a></div></div>
 +
</td></tr>
 +
<tr><td width='50%'>
 +
<div style="text-align:justify"><div class="thumbinner" width='100%'>
 +
<a href="https://static.igem.org/mediawiki/2011/b/b2/Aiia_HC_B0032.jpg">
 +
<img alt="" src="https://static.igem.org/mediawiki/2011/b/b2/Aiia_HC_B0032.jpg" class="thumbimage" width="100%"></a></div></div>
 +
</td>
 +
<td width='50%'>
 +
<div style="text-align:justify"><div class="thumbinner" width='100%'>
 +
<a href="https://static.igem.org/mediawiki/2011/d/d7/Aiia_HC_B0034.jpg">
 +
<img alt="" src="https://static.igem.org/mediawiki/2011/d/d7/Aiia_HC_B0034.jpg" class="thumbimage" width="100%"></a></div></div>
 +
</td></tr>
 +
</table>
 +
 +
 +
</p>
 +
</html>
 +
|}
 +
 
<!-- DON'T DELETE --><partinfo>BBa_K516222 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K516222 EndReviews</partinfo>

Revision as of 10:46, 23 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K516222

User Reviews

UNIQ7ce08b9fecb3bf6f-partinfo-00000000-QINU UNIQ7ce08b9fecb3bf6f-partinfo-00000001-QINU


UNIQ7ce08b9fecb3bf6f-partinfo-00000002-QINU

•••••

UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.

Characterized with:

The AiiA enzyme activity has been characterized under the regulation of ptet promoter, assaying its enzymatic activity.
The four measurement parts pTet-RBSx-AiiA-TT were assayed by BBa_T9002 biosensor.
No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0.
A significant HSL degradation was observed in E. coli TOP10 in high copy number plasmid pSB1A2.
In this case, a significant difference in degradation between pTet-RBSx-AiiA-TT and the negative control was observed, also just after 7 hours. That was the proof of the good functioning (in HIGH COPY) of the enzyme.

UNIQ7ce08b9fecb3bf6f-partinfo-00000005-QINU