Difference between revisions of "Part:BBa K590031"

Revision as of 01:40, 23 September 2011

Aldehyde Decarbonylase (ADC) - Synechococcus elongatus PCC7942

This part encodes an enzyme (Aldehyde Decarbonylase (ADC)) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. Works together with Acyl-ACP Reductase (AAR) to produce odd chain length alkanes. This part is an important component of the PetroBrick, which generates alkanes using AAR and ADC.

Usage and Biology

When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 730
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
-[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
-[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]]