Difference between revisions of "Part:BBa K676003:Design"

 
(Design Notes)
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<partinfo>BBa_K676003 short</partinfo>
 
<partinfo>BBa_K676003 short</partinfo>
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===Design Notes===
 
===Design Notes===
Design primer and perform PCR
 
  
 +
Primers designed for PCR:
 +
 +
Forward: TAGTTCGAATTCTCTAGACAGTTCATTTTTCTCAACG
 +
Reverse: ATCATCCTGCAGACTAGTACGGGGTAATGCTTTTTAC
  
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Final PCR product is 115 bp
  
 
===Source===
 
===Source===

Revision as of 20:53, 22 September 2011

TyrT Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers designed for PCR:

Forward: TAGTTCGAATTCTCTAGACAGTTCATTTTTCTCAACG Reverse: ATCATCCTGCAGACTAGTACGGGGTAATGCTTTTTAC

Final PCR product is 115 bp

Source

E Coli

References