Difference between revisions of "Part:BBa K590053:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Aldehyde Decarbonylase is transformed in PET29b+ vector with Kannamycin resistance. This | + | Aldehyde Decarbonylase is transformed in PET29b+ vector with Kannamycin resistance. This is PCR amplified and purified to extract ADC. This is double digested with XbaI and PstI. Later this is ligated with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 AAR-PSB1C3-High consititutive] that has been digested with SpeI and PstI. |
===Source=== | ===Source=== |
Revision as of 19:47, 22 September 2011
RrD_PSB1C3-High constitutive
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1584
Illegal XhoI site found at 2366 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Aldehyde Decarbonylase is transformed in PET29b+ vector with Kannamycin resistance. This is PCR amplified and purified to extract ADC. This is double digested with XbaI and PstI. Later this is ligated with AAR-PSB1C3-High consititutive that has been digested with SpeI and PstI.
Source
Gene-synthesis