Difference between revisions of "Part:BBa K590053:Design"

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===Design Notes===
 
===Design Notes===
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None
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Aldehyde Decarbonylase is transformed in PET29b+ vector with Kannamycin resistance. This was PCR amplified and purified to extract ADC. This was double digested with XbaI and PstI. Later this was ligated with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 AAR-PSB1C3-High consititutive] that has been digested with SpeI and PstI.
  
 
===Source===
 
===Source===

Revision as of 19:46, 22 September 2011

RrD_PSB1C3-High constitutive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1584
    Illegal XhoI site found at 2366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Aldehyde Decarbonylase is transformed in PET29b+ vector with Kannamycin resistance. This was PCR amplified and purified to extract ADC. This was double digested with XbaI and PstI. Later this was ligated with AAR-PSB1C3-High consititutive that has been digested with SpeI and PstI.

Source

Gene-synthesis

References