Difference between revisions of "Part:BBa K590054"
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When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. When mutant Aldehyde Decarbonylase and Acyl-ACP Reductase is co-expressed together, alkane production changed as seen on the right graph below. | When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. When mutant Aldehyde Decarbonylase and Acyl-ACP Reductase is co-expressed together, alkane production changed as seen on the right graph below. | ||
− | [[Image:Washinton_2011_Optimization_Quant.png|left|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details | + | [[Image:Washinton_2011_Optimization_Quant.png|left|450px|thumb|Wild type ADC and AAR are co-expressed and GC-MS quantification of this co-expression shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details]] |
[[Image:Washington_Redesign_Data2.png|right|430px|thumb|Mutant ADCs are co-expressed with AARs under optimized conditions.]] | [[Image:Washington_Redesign_Data2.png|right|430px|thumb|Mutant ADCs are co-expressed with AARs under optimized conditions.]] |
Revision as of 19:33, 22 September 2011
RrD_N25M-PSB1C3-High constitutive
This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.
Usage and Biology
When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. When mutant Aldehyde Decarbonylase and Acyl-ACP Reductase is co-expressed together, alkane production changed as seen on the right graph below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1584
Illegal XhoI site found at 2366 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]