Difference between revisions of "Part:BBa K590053"
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[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]] | [[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]] | ||
| − | [[Image:Washington_Redesign_Data2.png|450px|thumb|When this mutant ADC is co-expressed with AAR under optimized conditions,]] | + | [[Image:Washington_Redesign_Data2.png|left|450px|thumb|When this mutant ADC is co-expressed with AAR under optimized conditions,]] |
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Revision as of 19:07, 22 September 2011
RrD_PSB1C3-High constitutive
This part consists of Acyl-ACP Reductase, ribosomal binding site, Aldehyde Decarbonylase under the control of high constitutive promoter for alkane production.
Usage and Biology
When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. However, when mutant ADC is co-expressed with AAR, alkane profile is modified.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1584
Illegal XhoI site found at 2366 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]