Difference between revisions of "Part:BBa K606033"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K606033 short</partinfo> | <partinfo>BBa_K606033 short</partinfo> | ||
| − | T7 amber expression | + | T7 RNA polymerase tyrosine amber mutated expression casette. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The T7 RNA polymerases have two stops codons at the begening of its sequence. The protein cannot be expressed unless a tRNA amber supressor is expressed in the cell. (for instance K606034 for B. subtilis). This strategy can be use to create an and gate. If you want then the signal to be amplify, you can unse both K606018 or K606035 for reporting. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K606033 SequenceAndFeatures</partinfo> | <partinfo>BBa_K606033 SequenceAndFeatures</partinfo> | ||
Revision as of 17:06, 22 September 2011
pVeg SpoVG T7amber double terminator.
T7 RNA polymerase tyrosine amber mutated expression casette.
Usage and Biology
The T7 RNA polymerases have two stops codons at the begening of its sequence. The protein cannot be expressed unless a tRNA amber supressor is expressed in the cell. (for instance K606034 for B. subtilis). This strategy can be use to create an and gate. If you want then the signal to be amplify, you can unse both K606018 or K606035 for reporting.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]