Difference between revisions of "Part:BBa K606025"
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<partinfo>BBa_K606025 short</partinfo>
 
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YFP:tetR is a recombinant fusion protein. It is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO). Using the tetO array  (<partinfo>BBa_K606026</partinfo>) composed of a 10kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.<br> <br>Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)
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YFP:tetR is a recombinant fusion protein. It is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array  (<partinfo>BBa_K606026</partinfo>) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.<br> <br>Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The combination of both parts K606025 and K606026 can be use to concentrate a fluorescence signal and make a smaller nmber of fluorescent proteins visible under the microscope. See the experiments page for characterization and picture of what can be seen under the microscope.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K606025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K606025 SequenceAndFeatures</partinfo>

Revision as of 15:56, 22 September 2011

YFP:tetR recombinant fusion protein

YFP:tetR is a recombinant fusion protein. It is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array (BBa_K606026) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.

Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)

Usage and Biology

The combination of both parts K606025 and K606026 can be use to concentrate a fluorescence signal and make a smaller nmber of fluorescent proteins visible under the microscope. See the experiments page for characterization and picture of what can be seen under the microscope.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]