Difference between revisions of "Part:BBa J100032:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_J100032=== | ===Applications of BBa_J100032=== | ||
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| + | [[Image:proUP3.png]]<br> | ||
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| + | Tube A: E. coli + plasmids containing proUP3 (experimental)<br> | ||
| + | Tube B: E. coli + plasmids containing proUP3 + 20µL of 500mM NaCl (experimental #1 + inducer)<br> | ||
| + | Tube C: E. coli + plasmids containing the placeholder J100028 (negative control)<br> | ||
| + | Tube D: E. coli + plasmids containing pTet (positive control)<br> | ||
| + | Tube E: E. coli + plasmids containing pLux + 20µL HSL-3OC6 (experimental #2 + inducer)<br> | ||
| + | Tube F: E. coli + plasmids containing pLux (experimental #2)<br> | ||
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| + | The proUP3 promoter appeared to function, given the data in tube A above. However, contrary to expectations, it did not function in the presence of the osmotic inducer as shown in the data for tube B. This was likely due to the high concentration of NaCl supplied in addition to the LB, which already had a relatively high saline concentration. Further experimentation observing the promoter in the presence of varying concentrations of NaCl could be useful to understand more about this functioning promoter. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 13:52, 22 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J100032
Tube A: E. coli + plasmids containing proUP3 (experimental)
Tube B: E. coli + plasmids containing proUP3 + 20µL of 500mM NaCl (experimental #1 + inducer)
Tube C: E. coli + plasmids containing the placeholder J100028 (negative control)
Tube D: E. coli + plasmids containing pTet (positive control)
Tube E: E. coli + plasmids containing pLux + 20µL HSL-3OC6 (experimental #2 + inducer)
Tube F: E. coli + plasmids containing pLux (experimental #2)
The proUP3 promoter appeared to function, given the data in tube A above. However, contrary to expectations, it did not function in the presence of the osmotic inducer as shown in the data for tube B. This was likely due to the high concentration of NaCl supplied in addition to the LB, which already had a relatively high saline concentration. Further experimentation observing the promoter in the presence of varying concentrations of NaCl could be useful to understand more about this functioning promoter.
User Reviews
UNIQ14fc6506a11f3e72-partinfo-00000000-QINU UNIQ14fc6506a11f3e72-partinfo-00000001-QINU