Difference between revisions of "Part:BBa J100030:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_J100030=== | ===Applications of BBa_J100030=== | ||
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| + | All of the samples contained E. coli, growth media, and plasmids. Each of the columns is an average measure of fluorescence as a function of cell density from six different samples. In column 1, we had the phoA promoter. In column 2, we had the phoA promoter in addition to the inducer, a sugar named arabinose. However, the inducer for the phoA promoter is actually phosphate starvation, so the low fluorescene for column 2 does not indicate that the phoA promoter doesn't work to activate the Red Fluorescent Protein gene. In column 3, we had a negative control in the form of a DNA sequence called J100028, which is a placeholder insert to allow promoters to be ligated into plasmids. In column 4, we had the pTet promoter, the positive control known to activate the RFP gene. In column 5, we had the promoter pBad, along with its inducer arabinose (the same sugar inducer that we attempted to use for phoAO). In column 6, we had the promoter pBad but no inducer arabinose. | ||
===User Reviews=== | ===User Reviews=== | ||
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Revision as of 13:48, 22 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J100030
All of the samples contained E. coli, growth media, and plasmids. Each of the columns is an average measure of fluorescence as a function of cell density from six different samples. In column 1, we had the phoA promoter. In column 2, we had the phoA promoter in addition to the inducer, a sugar named arabinose. However, the inducer for the phoA promoter is actually phosphate starvation, so the low fluorescene for column 2 does not indicate that the phoA promoter doesn't work to activate the Red Fluorescent Protein gene. In column 3, we had a negative control in the form of a DNA sequence called J100028, which is a placeholder insert to allow promoters to be ligated into plasmids. In column 4, we had the pTet promoter, the positive control known to activate the RFP gene. In column 5, we had the promoter pBad, along with its inducer arabinose (the same sugar inducer that we attempted to use for phoAO). In column 6, we had the promoter pBad but no inducer arabinose.
User Reviews
UNIQ6a45ff1dc1f4247d-partinfo-00000000-QINU UNIQ6a45ff1dc1f4247d-partinfo-00000001-QINU