Difference between revisions of "Part:BBa K592011"
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'''iGEM11_Uppsala-Sweden:''' Expression of green chromoprotein. ''Escherichia coli'' constitutively expressing cjBlue (<partinfo>BBa_K592011</partinfo>).  
 
'''iGEM11_Uppsala-Sweden:''' Expression of green chromoprotein. ''Escherichia coli'' constitutively expressing cjBlue (<partinfo>BBa_K592011</partinfo>).  
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===References===
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[http://www.jbc.org/content/281/49/37813.abstract] Chan MC, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, Ikura M. (2006) ''Structural characterization of a'' ''blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus.'' J. Biol. Chem. 281(49). 37813-9
  
  

Revision as of 09:31, 22 September 2011

cjBlue, green chromoprotein

This chromoprotein from the Cnidopus japonicus sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to amilCP (BBa_K592009) and amilGFP (BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The sequence is codon optimized for expression in E coli.

Usage and Biology

This part is useful as a reporter.

CjBlue plate small.jpg

iGEM11_Uppsala-Sweden: Expression of green chromoprotein. Escherichia coli constitutively expressing cjBlue (BBa_K592011).


References

[http://www.jbc.org/content/281/49/37813.abstract] Chan MC, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, Ikura M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281(49). 37813-9


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]