Difference between revisions of "Part:BBa K590017:Experience"
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| − | We first extracted the individual genes as 4 inserts (down to a size that is more manageable), then we use Gibson Assembly method to put the inserts and the plasmid backbone together. The formation of cell chains can possibly be an indication of correct expression because the genes were reported to be important for chain alignments. | + | We first extracted the individual genes as 4 inserts (down to a size that is more manageable), then we use Gibson Assembly method to put the inserts and the plasmid backbone together. The formation of cell chains can possibly be an indication of correct expression because the genes were reported to be important for chain alignments. However, this has to be further studied. |
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Latest revision as of 07:33, 22 September 2011
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
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User Reviews
We first extracted the individual genes as 4 inserts (down to a size that is more manageable), then we use Gibson Assembly method to put the inserts and the plasmid backbone together. The formation of cell chains can possibly be an indication of correct expression because the genes were reported to be important for chain alignments. However, this has to be further studied.
UNIQa8ce234d66143f0d-partinfo-00000000-QINU UNIQa8ce234d66143f0d-partinfo-00000001-QINU