Difference between revisions of "Part:BBa K590063"

Revision as of 07:15, 22 September 2011

mamHIEJKL_pLacGFP_pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] using Gibson cloning method. It includes mamH, I, E, J, K, L all in pGA3k3_placGFP.

This part is contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome toolkit], it also serves as an evident that Gibson Cloning method is capable of combining multiple inserts.

Usage and Biology

We first extracted mamHI, mamE, mamJ, mamKL from the Magnetospirillum magneticum strain AMB-1, then we used Gibson cloning method to fuse the 4 groups of genes together to make a big HIEJKL insert. Followed by another gibson cloning reaction to combine the insert with the plasmid backbone pGA3k3_placGFP.

Below is the gel image of HIEJKL(~9000bp), we extracted this band and "gibsoned" it with pGA3k3_placGFP plasmid backbone.

1kb Ladder (left), mamHIEJKL (right)

Sequence and Features