Difference between revisions of "Part:BBa K590014"

Revision as of 06:41, 22 September 2011

pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

This is a Gibson Cloning friendly 3K3 plasmid backbone that was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] as part of the [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly toolkit].

Usage and Biology

This is a medium copy plasmid backbone which has Kanamycin resistance, with pLac promoter and GFP.

Below is the gel image of the plasmid backbone with 3555bp in length:

1kb Ladder (left), pGA3k3 (right)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
Illegal NheI site found at 1384
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
Illegal BglII site found at 2744
Illegal BamHI site found at 1
Illegal XhoI site found at 16
Illegal XhoI site found at 177
Illegal XhoI site found at 1020
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2729
Illegal AgeI site found at 1470
Illegal AgeI site found at 1793
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2498

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-This is a medium copy plasmid backbone which has Kanamycin resistance, with pLac promoter and GFP
+This is a medium copy plasmid backbone which has Kanamycin resistance, with pLac promoter and GFP.
+Below is the gel image of the plasmid backbone with 3555bp in length:
+[[Image:Igem2011 3k3 placGFP gel.png|thumb|center|1kb Ladder (left), pGA3k3 (right)]]
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