Difference between revisions of "Part:BBa K590008"

Latest revision as of 06:09, 22 September 2011

mamSTU, from Magnetospirillum magneticum

This part includes genes mamS, mamT, and mamU, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

mamS, mamT, and mamU are AMB0975, AMB0976, and AMB0977 respectively. Specific functions of mamS and mamU were not found. mamT was reported to be related to magnetite crystal growth; participates in different steps during magnetite synthesis.

Below is the gel image of the mamSTU (2030bp):

1kb Ladder (left), mamSTU (right)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1667
Illegal PstI site found at 437
Illegal PstI site found at 1949
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1667
Illegal PstI site found at 437
Illegal PstI site found at 1949
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1667
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1667
Illegal PstI site found at 437
Illegal PstI site found at 1949
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1667
Illegal PstI site found at 437
Illegal PstI site found at 1949
Illegal NgoMIV site found at 1005
Illegal NgoMIV site found at 1109
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2044

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
 mamS, mamT, and mamU are AMB0975, AMB0976, and AMB0977 respectively. Specific functions of mamS and mamU were not found. mamT was reported to be related to magnetite crystal growth; participates in different steps during magnetite synthesis.
+Below is the gel image of the mamSTU (2030bp):
+[[Image:Igem2011 STU gel.png|thumb|center|1kb Ladder (left), mamSTU (right)]]
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