Difference between revisions of "Part:BBa K590006"
 
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The genes are AMB0970 and AMB0971 respectively. It was reported that loss of mamP causes weak magnetic response, with large but fewer crystals; mamA is required for magnetosome activation; activation of vesicles
 
The genes are AMB0970 and AMB0971 respectively. It was reported that loss of mamP causes weak magnetic response, with large but fewer crystals; mamA is required for magnetosome activation; activation of vesicles
  
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Below is the gel image of mamPA(1493bp):
 +
[[Image:Igem2011 PA gel.png|center|thumb|1kb Ladder (left), mamPA (right)]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 06:04, 22 September 2011

mamPA, from Magnetospirillum magneticum

This part includes gene mamP and mamA, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

The genes are AMB0970 and AMB0971 respectively. It was reported that loss of mamP causes weak magnetic response, with large but fewer crystals; mamA is required for magnetosome activation; activation of vesicles

Below is the gel image of mamPA(1493bp):

1kb Ladder (left), mamPA (right)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 399
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 399
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 399
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 399
    Illegal NgoMIV site found at 269
    Illegal NgoMIV site found at 456
    Illegal NgoMIV site found at 1493
  • 1000
    COMPATIBLE WITH RFC[1000]