Difference between revisions of "Part:BBa K590002:Experience"
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The part is contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Background Magnetosome toolkit]. | The part is contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Background Magnetosome toolkit]. | ||
| − | It served as the template to amplify the Gibson Cloning friendly version of J to make [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590017 Part:BBa_K90017], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590020 Part: BBa_K590020]. The repetitive region in the middle of the mamJ sequence made it tricky to verify, we turned to the Komeili lab help, and we were suggested to use CGGTGTCCGTCTCCGA as the primer to verify the middle part of J sequence. | + | It served as the template to amplify the Gibson Cloning friendly version of J to make [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590017 Part:BBa_K90017], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590020 Part: BBa_K590020]. The repetitive region in the middle of the mamJ sequence made it tricky to verify, we turned to the Komeili lab help, and we were suggested to use CGGTGTCCGTCTCCGA as the primer to verify the middle part of J sequence. |
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| + | We also tried to fuse mamJ with fluorescent proteins on 1A3 backbone, although we could not verify the correct sequence, we observed some interesting expressions at the interesting images below. | ||
| + | [[Image:Igem2011 SfGFP-J-1A3-100iptg-01.jpg|left|300px]] [[Image:Igem2011 RFP-J-1A3-100iptg-02.jpg|center|300px]] | ||
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Revision as of 05:47, 22 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K590002
User Reviews
The part is contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Background Magnetosome toolkit]. It served as the template to amplify the Gibson Cloning friendly version of J to make Part:BBa_K90017, and Part: BBa_K590020. The repetitive region in the middle of the mamJ sequence made it tricky to verify, we turned to the Komeili lab help, and we were suggested to use CGGTGTCCGTCTCCGA as the primer to verify the middle part of J sequence.
We also tried to fuse mamJ with fluorescent proteins on 1A3 backbone, although we could not verify the correct sequence, we observed some interesting expressions at the interesting images below.
UNIQ10bdb66c4ec2a713-partinfo-00000000-QINU UNIQ10bdb66c4ec2a713-partinfo-00000001-QINU