Difference between revisions of "Part:BBa K606046:Experience"
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<h2>Re-cloning of KinA</h2> | <h2>Re-cloning of KinA</h2> | ||
We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had been sended to the registry into pSB1C3. | We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had been sended to the registry into pSB1C3. | ||
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<h2>Characterization of the sporulation induced by the expression of KinA</h2> | <h2>Characterization of the sporulation induced by the expression of KinA</h2> | ||
| − | <p>We used a strain holding a KinA gene under the control of a hyperspank promoter. Growing the cell into a synthetic minimal media for hours, we saw the cell starting | + | <p>We used a strain holding a KinA gene under the control of a hyperspank promoter. Growing the cell into a synthetic minimal media for hours, we saw the cell starting sporulating under the microscope after 3h. Here are the images from the characterization.</p> |
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Latest revision as of 04:50, 22 September 2011
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Applications of BBa_K606046
Re-cloning of KinA
We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had been sended to the registry into pSB1C3.
Characterization of the sporulation induced by the expression of KinA
We used a strain holding a KinA gene under the control of a hyperspank promoter. Growing the cell into a synthetic minimal media for hours, we saw the cell starting sporulating under the microscope after 3h. Here are the images from the characterization.
Ref: Single,chemically defined sporulation medium for bacillus subtilis: growth,sporulation,and extracellular protease production. James H.HAGEMAN et al
We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had been sended to the registry into pSB1C3.
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