Difference between revisions of "Part:BBa K606040:Experience"

(→‎Applications of BBa_K606040)
(→‎Applications of BBa_K606040)
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The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter thant the classic one, but is also recognised by E. coli polymerases.
 
The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter thant the classic one, but is also recognised by E. coli polymerases.
 
We cloned it first behind the amber tRNA that would induce a GFP amber on an other plasmid. Then to characterise it we cloned it behind an RFP, into TURBO cells that are LacIq to allow the promoter to be the less leacky possible.
 
We cloned it first behind the amber tRNA that would induce a GFP amber on an other plasmid. Then to characterise it we cloned it behind an RFP, into TURBO cells that are LacIq to allow the promoter to be the less leacky possible.
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+
<br><br>
 +
Without IPTG<br><br><br>
 
{| border="1" class="wikitable" style="text-align: center;"
 
{| border="1" class="wikitable" style="text-align: center;"
 
|E.coli containing Hyperspank+RBS+RFP - IPTG at 37°C
 
|E.coli containing Hyperspank+RBS+RFP - IPTG at 37°C
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|[[Image:minustrans2.jpg|450px|thumb|center|-IPTG E.coli at 37°C  (trans image)]]
 
|[[Image:minustrans2.jpg|450px|thumb|center|-IPTG E.coli at 37°C  (trans image)]]
 
|}
 
|}
−
 
+
<br>
 +
With IPTG : <br><br>
 
{| border="1" class="wikitable" style="text-align: center;"
 
{| border="1" class="wikitable" style="text-align: center;"
−
|E.coli containing Hyperspank+RBS+RFP - IPTG at 37°C
+
|E.coli containing Hyperspank+RBS+RFP + IPTG at 37°C
 
|-
 
|-
 
|[[Image:hovigfluo1.jpg|450px|thumb|center|+IPTG E.coli at 37°C  (rfp image)]]
 
|[[Image:hovigfluo1.jpg|450px|thumb|center|+IPTG E.coli at 37°C  (rfp image)]]

Revision as of 04:30, 22 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K606040

The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter thant the classic one, but is also recognised by E. coli polymerases. We cloned it first behind the amber tRNA that would induce a GFP amber on an other plasmid. Then to characterise it we cloned it behind an RFP, into TURBO cells that are LacIq to allow the promoter to be the less leacky possible.

Without IPTG


E.coli containing Hyperspank+RBS+RFP - IPTG at 37°C
-IPTG E.coli at 37°C (rfp image)
-IPTG E.coli at 37°C (trans image)
-IPTG E.coli at 37°C (rfp image)
-IPTG E.coli at 37°C (trans image)


With IPTG :

E.coli containing Hyperspank+RBS+RFP + IPTG at 37°C
+IPTG E.coli at 37°C (rfp image)
+IPTG E.coli at 37°C (trans image)
+IPTG E.coli at 37°C (rfp image)
+IPTG E.coli at 37°C (trans image)

User Reviews

UNIQa9f752436307bfba-partinfo-00000000-QINU UNIQa9f752436307bfba-partinfo-00000001-QINU