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| | __NOTOC__ | | __NOTOC__ |
| | <partinfo>BBa_K660201 short</partinfo> | | <partinfo>BBa_K660201 short</partinfo> |
| − | <h3>Illegal Restriction Sites</h3>
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| − | We are aware of two illegal restriction sites that exist in our latherin biobrick. Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for use with the Stratagene QuikChange Site-Directed Mutagenesis Kit.<br/><br/>
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| − |
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| − | <u>1st PstI site:</u><br/>
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| − | 5': GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/><br/>
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| − |
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| − |     Forward Primer: 5' CAG TTG CAG ACG GGT ATC TTG CAG TTT AAT TTC CGC<br/><br/>
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| − |
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| − |     Reverse Primer: 5' GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/><br/>
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| − |
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| − | MTP= 79.33 <br/><br/>
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| − |
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| − |
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| − | <u>2nd PstI site:</u><br/>
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| − | 5': GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/><br/>
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| − |
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| − |     Forward Primer: 5' G CGG ATT GCC GCT TTG CAG CTC AAT CGC<br/><br/>
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| − |
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| − |     Reverse Primer: 5' GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/><br/>
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| − |
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| − | MTP= 78.71<br/><br/>
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Latest revision as of 04:15, 22 September 2011
Latherin (Surfactant Protein) + 6xHIS Tag
