Difference between revisions of "Part:BBa K606046:Experience"

 
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===Applications of BBa_K606046===
 
===Applications of BBa_K606046===
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<h2>Characterization of the sporulation induced by the expression of KinA</h2>
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<p>We used a strain holding a KinA gene under the control of a hyperspank promoter. Growing the cell into a synthetic minimal media for for hours, we saw the cell starting soprulating under the microscope after 1h30. Here are the images from the characterization.</p>
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{| border="1" class="wikitable" style="text-align: center;"
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|+Sporulation assay with KinA overexpression
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|[[File:spor1.jpg|450px|thumb|center|Induction of sporulation after 3h on CDH Medium +4mM IPTG]]
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|[[File:spor2.jpg|450px|thumb|center|Induction of sporulation after 3h on CDH Medium +4mM IPTG]]
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|[[File:control spor.jpg|450px|thumb|center|Negative control of sporulation after 3h on CDH Medium +0mM IPTG]]
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<br>Ref: Single,chemically defined sporulation medium for bacillus subtilis: growth,sporulation,and extracellular protease production. James H.HAGEMAN et al
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<p>We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had bees sended to the registry into pSB1C3.</p>
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<p>This construct has been cloned right away into a replicative plasmid for subtilis and transformed, and we are caracterizing it at the moment.</p>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 04:10, 22 September 2011

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Applications of BBa_K606046

Characterization of the sporulation induced by the expression of KinA

We used a strain holding a KinA gene under the control of a hyperspank promoter. Growing the cell into a synthetic minimal media for for hours, we saw the cell starting soprulating under the microscope after 1h30. Here are the images from the characterization.


</html>

Sporulation assay with KinA overexpression
Induction of sporulation after 3h on CDH Medium +4mM IPTG
Induction of sporulation after 3h on CDH Medium +4mM IPTG
Negative control of sporulation after 3h on CDH Medium +0mM IPTG


Ref: Single,chemically defined sporulation medium for bacillus subtilis: growth,sporulation,and extracellular protease production. James H.HAGEMAN et al

We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had bees sended to the registry into pSB1C3.

This construct has been cloned right away into a replicative plasmid for subtilis and transformed, and we are caracterizing it at the moment.

===User Reviews=== BBa_K606046 StartReviews BBa_K606046 EndReviews