Difference between revisions of "Part:BBa K541545:Experience"
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We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein. | We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein. | ||
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===Applications of BBa_K541545=== | ===Applications of BBa_K541545=== | ||
Latest revision as of 02:58, 22 September 2011
THE SUICIDE EXPERIMENT OF E. COLI
Our K541545 part contains IPTG promoter and the gene part of LALF protein on a backbone which has resistance to chloramphenicol. We transported this part to E. coli for testing whether E. coli would kill itself or not.
Experiments of plates with and without IPTG
Plate A1: We streaked E coli culture which has K541545 on this plate and the plate includes IPTG and Chloramphenicol.
Plate A2: We streaked E coli culture which has K541545 on this plate and the plate includes just Chloramphenicol.
Plate B1: This plate does not have IPTG and we streaked E.coli culture which does not have IPTG promoter.
PlateB2: This plate has IPTG and we streaked E coli culture which has IPTG promoter.
We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein.
Applications of BBa_K541545
User Reviews
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